Five men wake up in a locked-down warehouse with no memory of who they are. They are forced to figure out who is good and who is bad to stay alive.

In 2006, in Red River, Tennessee, a teenager has frequent nightmares. Her mother reads an old letter from 1817 written by her ancestor, Lucy Bell. After a dispute of lands judged by the church, her husband John Bell is cursed by his opponent Kathe Batts, who has a fame of being a witch. From this moment on, an entity threatens John and her daughter Betsy Bell, attacking the girl during the nights. With the support of Betsy's school teacher Richard Powell, who tries to find rational explanations for the manifestation; her brother John Bell Jr.; and their friend James Johnston, who unsuccessfully tries to exorcize the entity from the house, the family does their best to protect Betsy in the haunted house.

While investigating a call in an abandoned house, Officer Frank Williams and a rookie find a woman brutally blinded, but they are attacked by a huge psychopath with an ax; the rookie is killed and Frank shots the criminal in the head, but has a severed arm. Four years later, the mutilated Frank is relocated, working as a guard in the County Detention Center. Frank goes with some delinquents to the Blackwell Hotel, an abandoned place since a fire burnt the last two floors, with the purpose of cleaning the location, preparing it to work as a shelter for homeless; in return, the criminals will have their sentences reduced. During the night, the inmate Kira that has some Christian tattoos in her body is kidnapped by the deranged serial-killer Kane that collect the eyes of his victims, while the rest of the group is attacked by the psychopath with his ax.

While recovering from a tragic accident on the road, the patrolman Edward Malus receives a letter from his former fiancée Willow, who left him years ago without any explanation, telling that her daughter Rowan is missing. Edward travels to the private island of Summerisle, where Willow lives in an odd community that plant fruits, and she reveals that Rowan is actually their daughter. Along his investigation with the hostile and unhelpful dwellers, Edward discloses that the locals are pagans, practicing old rituals to improve their harvest, and Rowan is probably alive and being prepared to be sacrificed. When he locates the girl, he finds also the dark truth about the wicker man.